Peptide Calculator — Reconstitution & Dosing
Calculate peptide reconstitution volumes, doses per injection, and syringe units. Includes BPC-157, TB-500, and custom peptide dosing for research purposes.
Research Use Only: Peptide calculators are provided for educational and research purposes only. Peptides are not approved by the FDA for human use outside of clinical trials. Consult a licensed medical professional before any administration.
| Peptide | Vial | Common Dose | Frequency | Route |
|---|---|---|---|---|
| BPC-157 | 5 mg | 200–500 mcg | Daily | SQ / IM |
| TB-500 | 2–5 mg | 2–2.5 mg | 2x/week | SQ / IM |
| Sermorelin | 3–9 mg | 200–300 mcg | Daily (night) | SQ |
| Ipamorelin | 2–5 mg | 200–300 mcg | 1–3x/day | SQ |
| CJC-1295 | 2–5 mg | 100–200 mcg | 2–3x/week | SQ |
| PT-141 | 10 mg | 1–2 mg | As needed | SQ / nasal |
| AOD-9604 | 1–2 mg | 300–500 mcg | Daily | SQ |
| GHK-Cu | 50–200 mg | 1–10 mg | Daily | SQ / topical |
Doses listed are from research literature. Not medical advice. Routes: SQ = subcutaneous, IM = intramuscular.
Frequently Asked Questions
- Use bacteriostatic water (BAC water) to dissolve the lyophilized (freeze-dried) peptide. Add BAC water slowly down the side of the vial — never inject directly onto the powder. Swirl gently, do not shake. Most peptides dissolve within seconds to minutes. Store reconstituted peptides refrigerated and use within 2–4 weeks typically.
- It depends on your desired concentration (dose per injection volume). Common approach: add 1–2 mL of BAC water per vial. Formula: BAC water (mL) = (Vial size in mg × 1000) ÷ (Desired mcg per injection) × injection volume in mL. Example: 5 mg BPC-157 vial, want 250 mcg per 0.1 mL → add 2 mL BAC water. Each 0.1 mL then contains 250 mcg.
- Bacteriostatic water (BAC water) contains 0.9% benzyl alcohol, which inhibits bacterial growth and preserves the peptide solution for up to 4 weeks once opened. Regular sterile water for injection is acceptable for single-use only. Tap water, saline, and distilled water should NOT be used for reconstitution — they may degrade the peptide or cause injection site irritation.
- Using a U-100 insulin syringe: if your concentration is 500 mcg/mL (0.5 mg/mL), a 100 mcg dose = 0.20 mL = 20 units on the syringe. Draw the calculated volume with a clean needle, inject subcutaneously (SQ) into the abdominal fold or outer thigh. For IM injections, use a longer needle (5/8" or 1"). Rotate injection sites.
- BPC-157 (Body Protection Compound) is a 15-amino-acid peptide derived from a gastric protein. Research doses: typically 200–500 mcg per day for healing protocols, administered SQ near the site of injury or IM. It's typically sold in 5 mg vials. Research suggests benefits for tendon, ligament, muscle, and gut healing in animal models.
- TB-500 is a synthetic version of Thymosin Beta-4, a 43-amino-acid peptide. Typical research protocol: 2–2.5 mg injected 2x per week for 4–6 weeks. TB-500 is generally used for systemic healing (acts further from injection site) vs. BPC-157 (which can be targeted locally). Some researchers combine both for synergistic effects.
- Reconstituted peptides should be stored refrigerated at 2–8°C (36–46°F) away from light. Most reconstituted peptides are stable for 2–4 weeks when refrigerated with BAC water. Lyophilized (dry) peptides stored properly can last 2+ years. Never freeze a reconstituted peptide — it may aggregate and lose potency.
- A U-100 syringe is calibrated for U-100 insulin (100 units per mL). 1 unit on a U-100 syringe = 0.01 mL. They're widely used for peptide injections because they have fine needles (27–31 gauge), hold small volumes precisely, and are inexpensive and widely available. Match syringe gauge to your injection route: 29–31G for SQ, 25–27G for IM.